Disciplinary keywords: microbial ecology; molecular biology; omics; biostatistical analysis; data integration
Topical keywords: bioprocesses; microbial biotechnologies; anaerobic digestion; waste valorisation; environmental samples
Lines of research:
- Methanization of ligno-cellulosic waste and biowaste, inhibition mitigation and process optimization
- Coupling of molecular microbiology and isotope biochemistry to study functional groups of microbes in complex ecosystems (Stable Isotope Probing, nanoSIMS, etc. )
- Applying metaomics methodologies to understand and optimize microbial processes (16S metabarcoding, metagenomics, metatranscriptomics, metabolomics)
- Deciphering omics data: omics data processing (Galaxy, QIIME, Python), statistical analyses of omics data (R), complex datasets integration (R)
PhD students supervision:
-Laëtitia Cardona (2017-2019)
-Simon Poirier (2013-2016): "Anaerobic digestion inhibitions: ecological dynamics, microbial indicators and resistance strategies. Examples of phenol and ammonia nitrogen"
Supervision of 10 master students or foreign PhD students during short stays in the lab
Ongoing and past projects
DIGESTOMIC ANR Project (2016-2020): "New operational strategies to overcome technical barriers in anaerobic digestion and extend its fields of application by using meta-omic approaches"
FASIC PHC Project (2017-2018): "Novel statistical methods to improve waste degradation: merging advanced biotechnologies with forefront analytical tools, identification of biomarkers by integrating multiple-omics datasets"
METHARESIST EC2CO Project (2015-2016): "Omics approaches to identify resistance strategies of anaerobic digesters to micropollutant : example of phenol"
Selected publications by date:
Madigou, C., Lê Cao, K.-A., Bureau, C., Mazéas, L., Déjean, S., Chapleur, O. 2019. Ecological consequences of abrupt temperature changes in anaerobic digesters. Chemical Engineering Journal 361:266-277.
Poirier S, Chapleur O. 2018. Influence of support media supplementation to reduce the inhibition of anaerobic digestion by phenol and ammonia: Effect on degradation performances and microbial dynamics. Data in Brief 19:1733-1754.
Poirier S, Madigou C, Bouchez T, Chapleur O. 2017. Improving anaerobic digestion with support media: Mitigation of ammonia inhibition and effect on microbial communities. Bioresour Technol 235:229-239.
Connan R, Dabert P, Le Roux S, Chapleur O, Bridoux G, Vanotti MB, Beline F, Magri A. 2017. Characterization of a combined batch-continuous procedure for the culture of anammox biomass. Ecological Engineering 106:231-241.
Poirier S, Bize A, Bureau C, Bouchez T, Chapleur O. 2016. Community shifts within anaerobic digestion microbiota facing phenol inhibition: Towards early warning microbial indicators? Water Res 100:296-305.
Poirier S, Desmond-Le Quéméner E, Madigou C, Bouchez T, Chapleur O. 2016. Anaerobic digestion of biowaste under extreme ammonia concentration: Identification of key microbial phylotypes. Bioresour Technol 207:92-101.
Hao L, Bize A, Conteau D, Chapleur O, Courtois S, Kroff P, Desmond-Le Quéméner E, Bouchez T, Mazéas L. 2016. New insights into the key microbial phylotypes of anaerobic sludge digesters under different operational conditions. Water Res 102:158-169.
Chapleur O, Madigou C, Civade R, Rodolphe Y, Mazéas L, Bouchez T. 2016. Increasing concentrations of phenol progressively affect anaerobic digestion of cellulose and associated microbial communities. Biodegradation 27:15-27.
Lü F, Bize A, Guillot A, Monnet V, Madigou C, Chapleur O, Mazéas L, He P, Bouchez T. 2014. Metaproteomics of cellulose methanisation under thermophilic conditions reveals a surprisingly high proteolytic activity. ISME J 8:88-102.
Chapleur O, Bize A, Serain T, Mazéas L, Bouchez T. 2014. Co-inoculating ruminal content neither provides active hydrolytic microbes nor improves methanization of 13C-cellulose in batch digesters. FEMS Microbiol Ecol 87:616-629.
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